Alginate-encapsulated muscle-derived stem cell spheroids promote muscle regeneration in a murine model of volumetric muscle loss.

ABSTRACT:

Introduction

Volumetric muscle loss (VML) remains a major clinical challenge due to the limited capacity of skeletal muscle to regenerate large-scale injuries. Muscle-derived stem cells (MDSCs) represent a promising therapeutic option for tissue regeneration; however, their clinical application is constrained by poor post-transplantation viability and limited engraftment. Alginate hydrogels offer a supportive three-dimensional microenvironment capable of encapsulating cells, promoting their survival, and enhancing paracrine signaling through the sustained release of growth factors.

Methods

In this study, we developed and characterized MDSC spheroids and evaluated their regenerative potential when encapsulated in RGD-modified alginate hydrogels. Co-culture with endothelial ce

SUBMITTER: Mitre LP 

PROVIDER: S-EPMC12740859 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature