Advanced Tissue Imprinting With Pneumatic Press for Mass Spectrometry Imaging of Plant Tissues.

ABSTRACT: Sample preparation is an important first step to obtain high quality mass spectrometry imaging (MSI) data. Preparing plant tissues is especially challenging for MSI of thin tissues along the lateral dimensions. The unique challenges involved with plant tissues, such as fragile cell walls, hydrophobic barriers, and specific tissue structures, often lead to inefficiency and difficulties in sample preparation. Imprinting plant tissues onto porous polytetrafluoroethylene (pPTFE) sheet has been widely used to extract internal metabolites in leaves and petals while keeping spatial resolution for MSI. However, pressure applications were typically made manually using a vise or pliers leading to low reproducibility and resolution in MS images. In this study, we introduce a home-built pneumatic pres

SUBMITTER: Hapuarachchige PNP 

PROVIDER: S-EPMC12741710 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature