Under pressure: integrated endothelial cell response to hydrostatic and shear stresses.

ABSTRACT: Blood flow within the vasculature is a critical determinant of endothelial cell (EC) identity and functionality, yet the intricate interplay of various hemodynamic forces and their collective impact on endothelial and vascular responses is not fully understood. Specifically, the role of hydrostatic pressure in the EC flow response is understudied, despite its known significance in vascular development and disease. To address this gap, we developed in vitro models to investigate how pressure influences EC responses to flow. Our study demonstrates that elevated pressure conditions significantly modify shear-induced flow alignment and increase EC density. Bulk and single-cell RNA sequencing analyses revealed that, while shear stress remains the primary driver of flow-induced transcriptional c

SUBMITTER: Mandrycky CJ 

PROVIDER: S-EPMC12741814 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature