RAA-CRISPR/Cas12a-driven two-tube, one-tube and one-tube-LFS for rapid detection of feline parvovirus.

ABSTRACT: A One-tube method biosensor was developed based on Two-tube recombinase-aid amplification (RAA)-CRISPR/Cas12a system due to the risk of aerosol contamination caused by open-tube operations in the Two-tube method. To further enable naked-eye visualization and portable detection of feline parvovirus (FPV), the lateral flow strip (LFS) technology was introduced to construct One-tube-LFS method. The limits of detection (LODs) of the One-tube and Two-tube methods were determined to be both 4.277 copies/μl, while the One-tube-LFS method was 42.77 copies/μl, which exhibited an LOD comparable to quantitative real-time polymerase chain reaction (qPCR). Notably, no cross-reactivity was observed with other common feline pathogens and the consistency of the three methods with qPCR results ranged from

SUBMITTER: Ge G 

PROVIDER: S-EPMC12741964 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature