Convergent Recruitment of Cerebellar Purkinje Cells by Mechanistically Diverse General Anesthetics.

ABSTRACT: General anesthesia produces a rapid and reversible loss of consciousness, yet the neural circuits through which chemically unrelated agents achieve this state remain largely unknown. Here, we combined Targeted Recombination in Active Populations (TRAP), Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC) tissue clearing, and light-sheet microscopy to generate a hemisphere-wide, single-cell atlas of neurons activated by three mechanistically distinct anesthetics in adult Fos^2A-iCreER/+; R26^Ai14/+ (TRAP2; Ai14) female mice. Automated alignment to the Atlas and unbiased effect-size analysis across 252 regions revealed a striking convergence. Vermal cerebellar lobules and their deep nuclei ranked at the top for every drug, whereas neocortic

SUBMITTER: Utsumi N 

PROVIDER: S-EPMC12742451 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature