A standard curve based method for relative real time PCR data processing.

ABSTRACT:

Background

Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may notably influence final results. The data processing is based either on standard curves or on PCR efficiency assessment. At the moment, the PCR efficiency approach is preferred in relative PCR whilst the standard curve is often used for absolute PCR. However, there are no barriers to employ standard curves for relative PCR. This article provides an implementation of the standard curve method and discusses its advantages and limitations in relative real time PCR.

Results

We designed a procedure for data processing in relative real time PCR. The procedure completely avoids PCR efficiency assessment, mini

SUBMITTER: Larionov A 

PROVIDER: S-EPMC1274258 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature