Reproducible Capillary Electrophoresis-Mass Spectrometry-Based Top-Down Proteomics of Complex Proteomes Enabled by an Advanced Cationic Polymer Coating.

ABSTRACT: Capillary zone electrophoresis-mass spectrometry (CZE-MS) has become a powerful tool for top-down proteomics (TDP). Capillaries are commonly coated with neutral or cationic polymers to reduce protein adsorption in CZE-MS. We recently introduced a cationic polymer coating [poly(acrylamide-co-(3-acrylamidopropyl) trimethylammonium chloride (PAMAPTAC)] to CZE-MS-based TDP. Here, we further improved the coating procedure to enhance the ease and repeatability of the cationic coating preparation by employing 2,2'-azobisisobutyronitrile (AIBN) as the radical initiator and methanol as the solvent. The new coating method not only simplifies the coating process but is also user-friendly. Different laboratory members were able to reproduce this type of cationic coating with consistent CZE-MS performa

SUBMITTER: Gao G 

PROVIDER: S-EPMC12742992 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature