Distinct Single-Cell Expression Pattern of the Soluble Epoxide Hydrolase and Cytochrome p450 Epoxygenases in Human and Mouse Small Intestinal Epithelia.

ABSTRACT: Lipid signaling molecules are essential for maintaining intestinal mucosal barrier homeostasis. Over 80% of polyunsaturated fatty acids (PUFAs) are metabolized through the cytochrome P450 epoxygenase (CYP)-soluble epoxide hydrolase (sEH) axis, generating bioactive epoxy and diol fatty acids. However, the expression patterns and functional roles of these enzymes in the intestinal epithelium remain poorly defined. To address this, we used RNAscope in situ hybridization and publicly available single-cell RNA sequencing (scRNAseq) datasets to map the cell-type-specific expression of PUFA-metabolizing enzymes in the small intestine. In humans, we identified three major epithelial expression patterns: (1) stem-cell-dominant (CYP2E1), (2) enterocyte-dominant (CYP1A1), and (3) widespread expressio

SUBMITTER: Sun L 

PROVIDER: S-EPMC12743005 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature