Impact of acetate on CO₂ fixation pathways in thermophilic and hydrogenotrophic bacteria.

ABSTRACT: The bacterial-type Wood-Ljungdahl (WL) pathway and reductive tricarboxylic acid (rTCA) cycle are the dominant chemolithotrophic CO₂ fixation pathways in bacteria inhabiting aphotic geothermal and deep-sea hydrothermal ecosystems. However, the activity of these bacterial metabolic systems in ecosystems with available organic carbons remains unclear. Here, we examined the impact of extracellular acetate on the CO₂-fixation pathways of three thermophilic hydrogen-oxidizing and non-acetogenic bacteria using ¹³C tracer-based metabolomics. Under chemolithoautotrophic conditions, Thermodesulfatator indicus and Hydrogenobacter thermophilus fixed CO₂ through the WL pathway and rTCA cycle, respectively, whereas Thermovibrio ammonificans

SUBMITTER: Chiba Y 

PROVIDER: S-EPMC12743299 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature