MiR-499 in Platelet-Derived Extracellular Vesicles Augments Inflammatory Cell Generation and Cardiac Remodeling After Myocardial Infarction.

ABSTRACT:

Background

Emergency myelopoiesis by bone marrow hematopoietic stem and progenitor cells (HSPCs) exacerbates disease pathology in various chronic diseases, including myocardial infarction (MI) and atherosclerosis. However, the mechanisms triggering myelopoiesis in the bone marrow after a distant organ injury, such as MI, remain unknown.

Methods

We ligated the left descending coronary artery to induce MI in mice. Platelet-derived extracellular vesicles (pEVs) were detected and enumerated in mice and patients with MI using NanoSight, ImageStream, and flow cytometry. microRNA in pEVs was quantified using a microRNA array. We used parabiosis, flow cytometry, adoptive transfer experiments, and transgenic mice to assess the effects of pEVs and microRNA on HSPC lineage commitment a

SUBMITTER: Ohayon-Steckel L 

PROVIDER: S-EPMC12743312 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature