Biocompatible SABRE Hyperpolarization of [1-¹³C]Ketoleucine for Cellular Metabolic Flux Sensing.

ABSTRACT: Ketoleucine (α-ketoisocaproate) is a novel hyperpolarized substrate for noninvasive metabolic imaging, enabling rapid, high-sensitivity detection of branched-chain amino acid flux, a pathway that is aberrant in many diseases including cancer and Alzheimer's disease. Utilizing NMR Signal Amplification by Reversible Exchange (SABRE) with an 80:20 acetone:water solvent system, we achieved >11% polarization of [1-¹³C]ketoleucine (corresponding to signal enhancement over 230,000-fold at 0.55 T) at 70 mM within 2 min, using parahydrogen as a cheap and fast source of hyperpolarization. A two-stage liquid-liquid extraction and gas stripping protocol removes excess excipients, yielding a biocompatible aqueous solution of [1-¹³C]ketoleucine (79 ± 10 mM). When mixed with Sacchar

SUBMITTER: TomHon P 

PROVIDER: S-EPMC12743355 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature