Impact of sperm fractionation on chromosome positioning, chromatin integrity, DNA methylation, and hydroxymethylation level.

ABSTRACT:

Background

Sperm chromosomes are nonrandomly organized in the cell nucleus, which plays an important role in the regulation of early embryo development, which is determined by the specific localization of sperm chromosomal regions carrying genes with expression crucial at the first contact with ooplasm during fertilization. Thus, the aim of this study is to determine whether the application of selective methods providing high-quality spermatozoa with good motility and/or morphology can increase the frequency of gametes with a specific positioning of chromosomes. For the first time, we used a sequential staining algorithm for consecutive analyses of the same individual spermatozoon with a fixed position, what enables one to achieve full and detailed documentation at the single cell

SUBMITTER: Graczyk Z 

PROVIDER: S-EPMC12743405 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature