The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation.

ABSTRACT:

Objective

Adipocyte differentiation is critical for the metabolically protective expansion of adipose tissue. Impaired differentiation drives lipodystrophy and pathologic tissue remodeling, major contributors to cardiometabolic diseases. The differentiation process is governed by master transcription factors, including the pioneer factor C/EBPβ, which initiates the adipogenic program. Here, we sought to identify novel C/EBPβ-associated factors that regulate human adipocyte differentiation.

Methods

We used chromatin immunoprecipitation followed by selective isolation of chromatin-associated proteins (ChIP-SICAP) to identify proteins that interact with C/EBPβ on chromatin during human adipocyte differentiation. Candidate factors were assessed for their effects on differentiati

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12743419 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature