Model-based evaluation of connexin hemichannel permeability.

ABSTRACT: Connexin (Cx) hemichannels form intercellular gap junction channels but can also function independently. These large-pore channels are permeable not only to ions but also to small signalling molecules and metabolites. This functional property is relevant to many physiological processes and can be altered by various biochemical factors or disease-causing mutations. In this study, we present a methodology for quantifying and comparing the permeabilities of hemichannels formed by different Cx isoforms using a combination of fluorescence imaging, electrophysiological recordings and mathematical modelling. Fluorescence imaging, coupled with mathematical modelling based on Fick's law and/or the Goldman-Hodgkin-Katz current equation, enables assessment of tracer diffusion rates. These data are in

SUBMITTER: Kraujalis T 

PROVIDER: S-EPMC12743422 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature