A Standard Operating Procedure for Protein Extraction From Abdominal Aortic Aneurysm Tissue: Enhancing Proteomics Applications.

ABSTRACT:

Purpose

The identification of putative biomarkers for AAA can be achieved through shotgun proteomics. However, tissue heterogeneity hampers its reproducible homogenization and protein extraction. Thus, we aimed to optimize a protocol to maximize protein yield and develop an SOP to foster reproducibility and accelerate translation of proteomics findings.

Experimental design

Using a bead-beating homogenization method, we compared the effect of beads' size, extraction cycles, beads-to-tissue mass ratio, lysis buffer volume, and chemistry on protein yield and/or qualitative and quantitative parameters of proteomics analysis (identifications, sequence coverage, coefficient of variation, functional enrichment analysis).

Results

Optimal conditions for protein extraction were

SUBMITTER: Baltazar T 

PROVIDER: S-EPMC12743587 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature