Preventing enzymatic degradation of demineralized dentin collagen using a natural crosslinker.

ABSTRACT:

Objectives

To evaluate effectiveness of cinnamaldehyde, vanillin and dialdehyde starch in stabilizing collagen in demineralized dentin against enzymatic degradation.

Materials and methods

Demineralized dentin collagen films were prepared from human teeth and treated for 3 min with 4% cinnamaldehyde, 4% vanillin or 4% dialdehyde starch. Deionized water and 4% glutaraldehyde served as negative and positive control. Crosslinker-collagen interaction was analysed using Fourier transform infrared spectroscopy (FTIR) (3 samples per group). After enzymatic degradation with collagenase type II, degradation was assessed via hydroxyproline assay (16 samples per group). Collagen network ultrastructure was examined using transmission electron microscopy (TEM) (2 samples per group). Data

SUBMITTER: Schestakow A 

PROVIDER: S-EPMC12743697 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature