Utilisation of mango seed husk for the production of phenolic compounds and glucose with C1184 enzyme preparation reveals the role of glucuronoyl esters in lignin-carbohydrate linkages in biomass recalcitrance.

ABSTRACT: This study assessed mango seed husk (MSH) fractions for producing glucose and phenolic compounds using commercial enzymes. We focused on cleaving lignin-carbohydrate linkages, specifically feruloyl and glucuronoyl esters, to decrease biomass recalcitrance and enhance product extraction. For saccharification studies, we used Sigma's C1184 cellulase from Aspergillus niger. Characterisation results of ground MSH using phloroglucinol and scanning electron microscopy revealed that it could be separated into a fine fraction, containing less lignin and cellulose fibres with parallel orientation, and a coarse fraction, with higher lignin content and cellulose fibres at an angled orientation. Activity assays and zymogram analysis of the C1184 preparation prior to saccharification studies revealed d

SUBMITTER: Mafa MS 

PROVIDER: S-EPMC12743729 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature