Global comprehensive transcriptomic and proteomic analyses of murine terminal erythroid differentiation.

ABSTRACT:

Background

Terminal erythroid differentiation (TED) is the maturation process of proerythroblasts into enucleated erythrocytes. Animal models are essential for studying red blood cell disorders.

Methods

We isolated erythroblasts at different TED stages from mouse bone marrow using FACS and performed integrated multi-omics analyses.

Results

We developed a stage-specific transcriptome and proteome profile, enhancing murine TED databases. The most significant changes occurred during the transition from proerythroblasts to basophilic erythroblasts, characterized by immune function suppression and activation of erythroid processes. Global gene and protein expression dynamics showed that orthochromatic erythroblasts exit the cell cycle, with transcription cofactors histone

SUBMITTER: Tang XY 

PROVIDER: S-EPMC12744273 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature