Role of miRNAs, miR-135b-5p & miR-21-5p in metastasis of cervical cancer.

ABSTRACT: Background & objectivesPersistent infection with high-risk human papillomaviruses (HPV) is a major cause of cervical cancer, inducing the hypoxic response by stabilising hypoxia inducing factor-1 alpha (HIF-1α). Under normoxia, HIF-1α is regulated by tumour suppressors genes, LIMD1 and VHL. This study aimed to elucidate the functional roles of two microRNAs, miR-135b-5p and miR-21-5p, in regulating LIMD1/ VHL and their impact on various cellular phenotypes relevant to progression of cervical cancer. MethodsExpressions of miR-135b-5p, miR-21-5p, LIMD1, and VHL was assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Target validation was performed via dual-luciferase assays. Functional assays (proliferation, migration, invasion, apoptosis, and cell cycle analysis) were

SUBMITTER: Sultana F 

PROVIDER: S-EPMC12744539 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature