Three PP2C phosphatases modulate FERONIA receptor kinase activity to regulate pollen-stigma interaction in Arabidopsis.

ABSTRACT: Pollen hydration represents the initial and critical step in pollen-stigma interactions and is necessary for successful plant fertilization. The FERONIA (FER) receptor kinase regulates pollen hydration by modulating stigmatic reactive oxygen species (ROS) accumulation through rapid alkalinization factor 23/33 (RALF23/33) and pollen coat protein B-class peptide (PCP-B) signaling. However, the function and regulatory mechanism of FER's receptor kinase activity in pollen hydration remain poorly understood. In this study, we found that the kinase-dead form of FER^K565R fails to restore stigmatic ROS accumulation and pollen hydration in the fer-4 mutant. By integrating RNA sequencing database analyses with yeast two-hybrid assays, we identified three type 2C phosphatases (PP2Cs)-prote

SUBMITTER: Cheng L 

PROVIDER: S-EPMC12744760 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature