Multi-Omics Analysis Reveals Photodynamic Therapy Ameliorating Skin Photoaging by Improving Cellular Senescence Through Mitohormesis-Mediated Reduction of Citrate Content.

ABSTRACT: Clinical evidence supports the anti-photoaging efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT), yet its mechanism remains elusive. Paradoxically, ALA-PDT generates reactive oxygen species (ROS), a key mediator of ultraviolet radiation (UVR)-induced photoaging, raising questions about its rejuvenating effects. Here, we employed a multi-omics approach to clarify this paradox. A UVR-induced hairless mouse model of photoaging was treated with ALA-PDT, followed by transcriptomic, proteomic, and metabolomic profiling of skin biopsies. In vitro, fibroblast senescence was induced by UV irradiation to evaluate ALA-PDT's protective effects. Mitochondrial function and citrate (CA) levels were assessed pre- and post-treatment. ALA-PDT significantly ameliorated photoaging phenotypes in

SUBMITTER: Yan Y 

PROVIDER: S-EPMC12744960 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature