Epitope-level analysis of cross-reactive human HLA antibodies against genetically modified swine leukocyte antigens in xenotransplantation.

ABSTRACT:

Introduction

Xenotransplantation using genetically modified pigs is a promising solution to organ shortages, particularly for highly sensitized patients with broad anti-HLA sensitization who lack compatible allografts. However, preformed human anti-HLA antibodies may cross-react with porcine SLA, posing a barrier for clinical application. This study aims to characterize the extent and specificity of cross-reactive antibody responses against genetically engineered pig PBMCs, particularly from quadruple knockout (QKO) pigs.

Methods

We evaluated antibody binding and cytotoxicity of 68 human sera stratified by HLA class I and II antibody profiles using flow cytometric crossmatch (FCXM), complement-dependent cytotoxicity (CDC) assays (CDC-NIH and CDC-AHG), antibody elution, and s

SUBMITTER: Cho SI 

PROVIDER: S-EPMC12745215 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature