High Shear Stress-Induced Endothelial Piezo1 Downregulation Promotes Intracranial Aneurysm Formation via the PDGF-BB/PDGFRβ Paracrine Signaling Pathway.

ABSTRACT:

Background

Abnormally high shear stress (HSS) is strongly associated with intracranial aneurysm (IA) formation. Endothelial Piezo1 is sensitive to shear stress stimulation, but the mechanism by which it mediates this mechanobiological coupling process is unclear.

Methods

The correlation between shear stress and the Piezo1 expression was investigated using human IA samples and a parallel-plate flow chamber system. To determine the effects of endothelial Piezo1 on the phenotype of neighboring vascular smooth muscle cells (VSMCs) and IA formation, the CRISPR/Cas9 system was used to inhibit endothelial Piezo1 gene expression in vitro. Piezo1^ΔEC mice were produced by injecting AAV2-BR1-Tie2-Cre into 8-week-old male Piezo1^flox/flox mice, which were further us

SUBMITTER: Lu Z 

PROVIDER: S-EPMC12745340 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature