Not Aging but Calorie Restriction Strongly Affects Protein Oxidation in Heart and Brain Mitochondria.

ABSTRACT: Aging is an inevitable consequence for all organisms. According to the mitochondrial free radical theory of aging (MFRTA), reactive oxygen species (ROS), which are predominantly generated in mitochondria, are assumed to play a key role. Calorie restriction (CR) delays aging by improving mitochondrial function; however, the molecular mechanisms underlying the effects of ROS and CR on mitochondria remain poorly understood. Oxidative protein modifications in mitochondrial proteins from the heart and cerebrum of young (6.5 months) and old (27 months) rats were quantified and the effects of short-term and lifelong CR interventions were investigated. Mass spectrometry was leveraged to achieve an unbiased and comprehensive analysis of various types of oxidative postranslational modifications (oxP

SUBMITTER: Fan S 

PROVIDER: S-EPMC12745344 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature