Functional and Structure Prediction of Hypothetical Proteins From <i>Listeria aquatica</i> FSL S10-1188: Bioinformatics Approaches.

ABSTRACT:

Background

Listeria aquatica FSL S10-1188 is a freshwater bacterium characterized by distinctive metabolic properties and a genome enriched in hypothetical proteins (HPs). Although it is not considered pathogenic, its genomic features and aquatic habitat raise concerns about potential involvement in horizontal antimicrobial resistance (AMR) gene transfer.

Methods

The genome of L. aquatica FSL S10-1188 (GenBank Accession: CP011539.1) contains 2789 predicted proteins that were initially screened. From these, 919 HPs were prioritized based on sequence length (> 50 amino acids), physicochemical stability assessed using ProtParam, and consistent subcellular localization predictions from CELLO, PSORTb, and PSLpred. Among these, two stable cytoplasmic, HPs EUJ18943.1

SUBMITTER: Abdallah MM 

PROVIDER: S-EPMC12745843 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature