Homonuclear Chemical Shift Correlation in Solids Under MAS by Fast Cross-Relaxation Driven Spin Diffusion.

ABSTRACT: In this study, we report a two-dimensional NMR technique that correlates the chemical shifts of homonuclear spin systems in solids under MAS. The pulse sequence employs double spin-lock RF pulses to facilitate magnetization exchange among low-γ nuclei (such as ¹³C or ¹⁵N) through cross-relaxation driven by a combination of spin diffusion and RF field. We systematically investigate how the efficiency of the magnetization exchange depends on the Hartmann-Hahn mismatch and the MAS frequency. Experimental results obtained from ¹³C-labeled Fmoc-Leucine powder sample, ¹⁵N-labeled L-histidine amino acid (pH 6.3) powder sample, and uniformly-¹⁵N-labeled aquaporin reconstituted in DMPC lipid vesicles are reported. The results reveal a rapid spi

SUBMITTER: Fu R 

PROVIDER: S-EPMC12746031 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature