Growth-coupled selection of 5-aminolevulinic acid synthetase mutants for 5-aminolevulinic acid biosynthesis in <i>Corynebacterium glutamicum</i>.

ABSTRACT:

Introduction

5-Aminolevulinic acid (5-ALA) is a nonprotein amino acid with broad applications in agriculture, medicine, and the food industry. In recent years, substantial efforts have been devoted to enhancing its biosynthesis. The catalytic activity of 5-aminolevulinic acid synthase (ALAS) is a critical determinant of production efficiency.

Methods

In this study, a growth-coupled selection strategy was developed to improve ALAS from Rhodobacter capsulatus SB1003 using random and site-specific mutagenesis. The derived ALAS mutant was then introduced into the GRAS-certified producer Corynebacterium glutamicum, followed by fermentation optimization.

Results

The enzymatic activity of the best mutant, D4,7,18, increased by 67.41%, leading to 1.18-fold highe

SUBMITTER: Yang P 

PROVIDER: S-EPMC12746484 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature