Evaluation of the reproducibility and performance characteristics of the Phagomagnetic separation-qPCR assay for rapidly detecting viable <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> in bovine milk and feces.

ABSTRACT: Inter-laboratory trials were carried out to evaluate the reproducibility and estimate test performance characteristics of the Phagomagnetic separation (PhMS)-qPCR assay, a novel phage-based assay recently developed as a rapid alternative to culture for detecting viable Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk and feces. Unique reagents and a detailed instruction manual required for the PhMS-qPCR assay were provided to five European veterinary diagnostic laboratories by Rapid-Myco Technologies Limited. Milk and feces test panels were prepared at NEIKER and distributed to participant laboratories between April-June 2023 and March-May 2024, respectively. Each test panel comprised of MAP-spiked and/or naturally infected bovine milk or feces samples (18 sam

SUBMITTER: Grant IR 

PROVIDER: S-EPMC12746688 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature