ETV1 is a key regulator of enteroendocrine PYY production.

ABSTRACT: The intestine is a rich source of hormones that regulate metabolism. Among these are glucagon-like peptide-1 (GLP-1) and peptide YY (PYY), both expressed by L-cells. These hormones play important roles in promoting satiety; however, how they are regulated transcriptionally is not known. ETS variant transcription factor 1 (ETV1) is expressed by L-cells, but its function remains unknown. Here, we examined Etv1 expression in single-cell RNA-sequencing (scRNA-seq) datasets from the mouse small intestine and from organoid cultures. To assess the functional role of ETV1, loss-of-function and overexpression experiments were performed in organoids. Gene expression was subsequently assessed with quantitative PCR and scRNA-seq. Our results confirmed Etv1 enrichment in the L-cell lineage both in vivo

SUBMITTER: Baattrup AM 

PROVIDER: S-EPMC12746708 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature