Microtubule dependent changes in cell polarity are required for mammary epithelial cell migration and branching morphogenesis.

ABSTRACT: Branching morphogenesis requires dramatic changes in cell polarity, proliferation, migration, and actin dynamics to elaborate tubular networks. However, little is known about how microtubules support these cell behaviors in 3D tissues. Using organotypic cultures, we first examined the organization of the microtubule cytoskeleton. Simple luminal epithelial cells exhibited non-centrosomal, apico-basally oriented microtubule arrays, while stratified luminal cells had centrosomally radiating microtubules. During collective migration, luminal cells adopted an ameboid-like organization with a rear-facing nuclear-centrosomal axis. Multiple staining approaches suggest that cells in the basal-most luminal cell layer had more stable microtubules than cells deeper within the stratified layer. Finally

SUBMITTER: Fraser AK 

PROVIDER: S-EPMC12746711 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature