In C57BL/6J mice, weight loss in previously obese mice reduced bone mass and shifted the cortical bone metabolome.

ABSTRACT: Obesity is linked to increased fracture risk. Despite the negative effects of weight loss on the skeleton, patients with obesity are advised to lose weight via calorie restriction. Obesity and weight loss individually alter both whole-body and local metabolism. Little is known about changes to bone mass and metabolome following calorie restriction in obese preclinical models. We hypothesized that caloric restriction would reduce bone mass in obese mice and would alter the cortical bone metabolome. To induce obesity, 8-week-old male and female C57BL/6J mice received 60 % kCal high-fat diet for 12 weeks. From 20 to 30 weeks of age, mice either remained obese or lost weight through 30 % caloric restriction. Controls consumed 10 % kCal low-fat diet. Compared to obesity, calorie restriction eli

SUBMITTER: Chlebek C 

PROVIDER: S-EPMC12746795 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature