Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging.

ABSTRACT: Single-molecule localization microscopy (SMLM) is a powerful tool for observing structures beyond the diffraction limit of light. Combining SMLM with engineered point spread functions (PSFs) enables 3D imaging over an extended axial range, as has been demonstrated for super-resolution imaging of various cellular structures. However, super-resolving structures in 3D in thick samples, such as whole mammalian cells, remains challenging as it typically requires acquisition and postprocessing stitching of multiple slices to cover the entire sample volume or more complex analysis of the data. Here, we demonstrate how the imaging and analysis workflows can be simplified by 3D single-molecule super-resolution imaging with long-axial-range double-helix (DH)-PSFs. First, we experimentally benchmark

SUBMITTER: Nakatani Y 

PROVIDER: S-EPMC12747171 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature