Enhancing Mycoplasma synoviae control: A novel quantitative real-time PCR approach to differentiate MS-H vaccine strain and field strains.

ABSTRACT: Mycoplasma synoviae (MS) is a prevalent pathogen in poultry, causing substantial economic losses. Vaccination is a crucial strategy for controlling MS infections. In China, a temperature-sensitive live vaccine, Vaxsafe®MS (MS-H strain), was introduced to address the rising prevalence of MS since 2017. Accurate differentiation between the MS-H vaccine strain and field strains is essential for the effective application of the vaccine and the eradication of MS in poultry. In this study, a duplex TaqMan real-time quantitative PCR (qPCR) method was first developed based on a single nucleotide polymorphism (SNP) at position 367 of the obg gene. However, some reisolated strains of the MS-H vaccine strain may exhibit reverse mutations at this site, potentially leading to false-positive results fie

SUBMITTER: Chen M 

PROVIDER: S-EPMC12747186 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature