Functional and transcriptional responses of chicken macrophages to hypoxia.

ABSTRACT: The emergence of wooden breast (WB) myopathy, marked by palpable hardness and excessive fibrosis in the affected pectoralis major muscle of broilers, has greatly affected poultry industry. Hypoxia-triggered dysfunction of macrophages play a crucial role in inflammation-driven tissue fibrosis. Nevertheless, how hypoxia-induced changes in chicken macrophages contribute to WB development remain poorly understood. In this study, we established an in vitro hypoxia model using chicken macrophages HD11 cells and characterized their functional and transcriptional alterations. The results demonstrated that hypoxia enhanced macrophage proliferation while suppressing their migration. RNA-seq analysis revealed 418, 1,462, and 2,670 differentially expressed genes (DEGs) at 12 h, 24 h, and 48 h post-hyp

SUBMITTER: Li D 

PROVIDER: S-EPMC12747202 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature