Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow.

ABSTRACT: During mouse development, hematopoietic stem and progenitor cells (HSPC) originate from hemogenic endothelial cells (ECs) through a process of endothelial-to-hematopoietic transition. These HSPC are thought to fully sustain adult hematopoiesis. However, it remains unknown whether adult ECs retain hemogenic potential. Here we used in vivo genetic lineage tracking at population and single-cell (sc) levels, scRNA sequencing, and bone marrow transplantation to detect hemogenic ECs in adult mice. We identify and characterize a small population of bone marrow-resident, adult Cdh5/VE-Cadherin⁺ ECs that produce hematopoietic cell-progeny in vitro and in mice. These adult hemogenic ECs and their hematopoietic cell progeny can give rise to hematopoietic cells following adoptive tra

SUBMITTER: Feng JX 

PROVIDER: S-EPMC12747260 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature