A 38-plex PCR MALDI-TOF MS-based assay to detect SNPs common in elite athletes.

ABSTRACT: There is great demand for a novel technique to facilitate the rapid identification of multiple single-nucleotide polymorphisms (SNPs) prevalent in elite athletes. Case-control and genome-wide association studies (GWAS) have been conducted to investigate an individual's likelihood for success in various sports, revealing several putative loci associated with elite athletic status. However, it remains challenging to detect multiple such SNPs simultaneously with the aim of examining their influence on specific physical fitness characteristics, such as muscle power, strength, or endurance. The aim of the present study is to develop a 38-plex PCR amplification assay, integrated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), for the identificati

SUBMITTER: Zannah M 

PROVIDER: S-EPMC12747323 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature