Characteristics of spatiotemporal distribution of HIV-1 Gag-containing complexes on the dorsal membrane tracking with live confocal imaging.

ABSTRACT:

Introduction

In recent years, novel detection methods have provided significant insights into the real-time morphological changes occurring during the HIV life cycle within host cells. However, the detailed dynamics of virus assembly and release, particularly from the perspective of the dorsal cell membrane, remain poorly understood.

Methods

HEK293T cells were transfected with pEGFP-N3-Gag plasmids, and the spatiotemporal distribution of Gag-EGFP was monitored using the "xyt" or "xyz" imaging modes of laser confocal microscopy (LCM). The motion trajectory of GCC in living cells was manually tracked using the MtrackJ plugin in ImageJ 1.54p. 3D reconstruction of target proteins were processed with the Volume Viewer plugin in ImageJ 1.54p.

Results

The results revealed th

SUBMITTER: Wang X 

PROVIDER: S-EPMC12747361 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature