A methodology to reduce the localization error in multi-loci microscopy provides new insights into enhancer biology.

ABSTRACT: Numerous functions hinge on the spatial arrangement of different genomic loci. Hence, microscopy techniques, such as chromatin tracing, have been developed to localize multiple loci in fixed cells. Depending on the throughput and specifics of the experiment, localization errors can still obscure the true spatial locations. We have developed a post-processing methodology to address this challenge without the need for additional experimentation: Loci Enabled Advanced Resolution (LEAR). By leveraging the fact that localization errors increase the variability of the displacements between loci, and given an approximation of the localization error, we can approximate the ground truth spatial variation for each pair of loci to guide an iterative error correction process. After validating our appr

SUBMITTER: Bohrer CH 

PROVIDER: S-EPMC12747437 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature