Decitabine promotes degradation of DNMT1 and EZH2 via the ubiquitination pathway and inhibits colorectal cancer progression.

ABSTRACT:

Purpose

This study aimed to investigate the role of DNMT1 in CRC progression and its regulatory relationship with TRAF6 and EZH2.

Methods

DNMT1 expression was analyzed in CRC tissues and cell lines using public databases and experimental techniques, including Western blot and qRT-PCR. Functional assays, such as colony formation, transwell migration/invasion, and cell cycle analysis, were performed to assess the role of DNMT1 in CRC cell proliferation and metastasis. Mechanistic studies, including cycloheximide (CHX) chase assays, ubiquitination assays, and co-immunoprecipitation (Co-IP), were conducted to explore the regulation of DNMT1 stability and its effects on EZH2 protein stability. The regulatory axis was further validated using methylation-specific PCR (MSP), dual-lu

SUBMITTER: Peng XM 

PROVIDER: S-EPMC12748097 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature