IL-18 receptor axis in allergic conjunctivitis: a multi-omics study.

ABSTRACT:

Purpose

The pathophysiology of allergic conjunctivitis (AC) is not fully explained by the traditional Th2-centric model. This study aimed to identify AC-associated candidate genes and delineate immune pathways, with a focus on cell-type-specific mechanisms that could contribute to disease heterogeneity.

Methods

We employed an integrative multi-omics strategy, using three genomic analyses (FUSION, MAGMA, UTMOST) on human GWAS data to identify AC-associated candidate genes. These candidates were then investigated using single-cell RNA sequencing data from a mouse model of AC to map immune cell communication and signaling dynamics. Key pathways were validated in an independent ovalbumin-induced AC mouse model using clinical scoring, qRT-PCR and Western blot analysis.

Results

SUBMITTER: Yuan L 

PROVIDER: S-EPMC12748174 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature