Exploring targeted therapies against RFPL3 for non-small cell lung cancer with lymph node metastasis.

ABSTRACT:

Background

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-related deaths worldwide, marked by its aggressive nature and high likelihood of metastasis. The precise function of ret finger protein-like 3 (RFPL3) in NSCLC progression remains incompletely understood.

Methods

This study measured RFPL3 expression in NSCLC by immunohistochemistry, qPCR, and Western blot. RFPL3's effects on proliferation were assessed with CCK-8 and colony formation assays. Transwell and wound-healing assays evaluated its role in invasion, metastasis, and EMT. In vitro and in vivo studies determined RFPL3's mechanisms driving EMT and lymphatic metastasis.

Results

RFPL3 was overexpressed in NSCLC tissues, correlating with lymphatic metastasis and poor prognosis, estab

SUBMITTER: Lin C 

PROVIDER: S-EPMC12748340 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature