Single-cell aggrephagy landscape delineates intercellular communication of tumor microenvironment associated with ovarian cancer progression and immunotherapy.

ABSTRACT: Aggrephagy plays a pivotal role in ovarian cancer (OC) progression by clearing misfolded proteins. However, the functional role of aggrephagy within the tumor microenvironment (TME) remains elusive. Non-negative matrix factorization (NMF) was employed to analyze 43 aggrephagy-related genes (ARGs) on 65,820 single cells from scRNA-seq data of 12 samples (7 OC tissues and 5 normal tissues). The OC cohort from 9 public databases and TIDE-based immunotherapy scores were used to identify prognosis and immune response patterns of these TME cell clusters. Furthermore, the association between ARGs and CD8+ T cells was validated by the ID8 ovarian cancer mouse subcutaneous tumor model. Fibroblasts, T cells, macrophages and B cells identified from scRNA-seq of OC samples were subjected to biological

SUBMITTER: Fang F 

PROVIDER: S-EPMC12748359 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature