Multi-omics analysis reveals PIAS family gene dysregulation as a driver of head and neck squamous cell carcinoma (HNSC) progression and therapeutic vulnerability.

ABSTRACT:

Background

Head and neck squamous cell carcinoma (HNSC) stand as one of the most fatal malignancies worldwide. The intricate nature of this disease has contributed to a limited understanding of its diagnostic and prognostic indicators. This study comprehensively investigated the dysregulation of PIAS family genes (PIAS1, PIAS2, PIAS3, and PIAS4) in Head and Neck Squamous Cell Carcinoma (HNSC).

Methods

Cell culturing, RT-qPCR, bisulfite sequencing, UALCAN, GEPIA, Human Protein Atlas (HPA), MEXPRESS, cBioPortal, Prognostic model development, DAVID, TISCH2, cell culture, gene knockdown, cell culture, colony formation, wound healing, DrugBank, and molecular docking analyses.

Results

RT-qPCR demonstrated significant up-regulation of PIAS1, PIAS2, PIAS3, and PIAS4 in HNSC c

SUBMITTER: Hussain M 

PROVIDER: S-EPMC12748365 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature