Peptide antibody tools to dissect specific functions of eIF4A paralogs in cancer.

ABSTRACT: Eukaryotic translation initiation factors (eIFs) are key regulators of messenger RNA (mRNA) translation, a process highly dysregulated in cancer. Among these paralogs, eIF4A1 and eIF4A2 are closely related DEAD box mRNA helicases that, despite their high sequence identity, play distinct roles in cellular homeostasis and tumorigenesis. In the context of triple-negative breast cancer (TNBC), where dysregulated protein translation drives tumor progression and metastasis, high rate of relapse, and resistance to radio- and chemotherapy, an understanding of the unique contributions of eukaryotic initiation factor 4 A (eIF4A) paralogs is important. Current commercial antibodies are not validated to claim the specificity towards these helicases. To address this, we developed and validated paralog-

SUBMITTER: Srivastava S 

PROVIDER: S-EPMC12748412 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature