Identification of ITGA2 as a methylation-regulated oncogene through a CeRNA network in papillary thyroid carcinoma.

ABSTRACT:

Objective

Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. This study aimed to construct a competing endogenous RNA (ceRNA) network to identify potential molecular targets and validate their biological functions.

Methods

Gene expression profiles from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases were integrated to construct a miRNA-mediated mRNA-mRNA network within a ceRNA framework. Pathway enrichment analysis was applied to identify candidate genes. Differential expression in clinical specimens was validated by Western blot and qRT-PCR. Functional assays were performed after target gene silencing in PTC cell lines. Methylation specific PCR (MSP) was used to assess the relationship between promoter methylation and g

SUBMITTER: Wu G 

PROVIDER: S-EPMC12748424 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature