Developing a theranostic nanobody targeting FAP for cancer imaging and therapy.

ABSTRACT:

Background

Fibroblast activation protein (FAP) is a pan-cancer target. Its selective expression on the majority of solid tumors with minimal to absent expression in healthy tissues positions FAP as a promising target for radiotheranostic applications. Nanobodies (Nbs) have unique characteristics, including small size, high affinity, stability, and ease of modification, making them ideal candidates for cancer diagnostics and targeted radiotherapeutics. Llama-derived Nbs were generated and screened against full-length FAP, with three unique candidates selected from the library for further characterization. The lead candidate Nb159 was engineered for site-specific radiolabeling with ⁸⁹Zr for PET imaging and with ¹⁷⁷Lu coupled with PEG for therapeutic evaluation i

SUBMITTER: Ben-Naim L 

PROVIDER: S-EPMC12748472 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature