Probiotic-based oral vaccine mucosal delivery system enabling genetically encoded dual-antigen arrays.

ABSTRACT: Oral vaccines provide a non-invasive approach for cancer immunotherapy but face challenges in gastrointestinal stability, antigen presentation, and mucosal delivery. Here, we present an engineered probiotic-based oral vaccine system, Bac^{OR-Fn-T+phiX174}, featuring genetically encoded dual-antigen ferritin arrays and inducible bacterial lysis. Upon oral administration and arabinose induction, the probiotic strain lyses in situ, releasing OVA/TRP2-decorated ferritin nanoparticles that efficiently traverse the intestinal barrier via M-cell targeting and activate mucosal dendritic cells. This platform robustly stimulates CD8⁺ and CD4⁺ T-cell responses, enhances B-cell and macrophage activation, reduces regulatory T cells, and provides therapeutic efficacy agains

SUBMITTER: Yue Y 

PROVIDER: S-EPMC12748624 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature