Single-cell RNA sequencing revealed the association between proximal tubular epithelial cells and renal interstitial cells in chronic kidney disease progression.

ABSTRACT: Proximal tubular epithelial cells (PTECs) injury is a driving force toward chronic kidney disease (CKD) progression. However, the characteristics of injured PTECs during CKD pathogenesis and their mechanisms of coordination with specific interstitial cells to promote disease progression require further elucidation. In this study, CKD mouse models were established by administering a 0.2% adenine-containing diet for 2, 4, and 8 weeks to simulate progressive CKD. Renal injury was assessed through histopathology, serum creatinine (Scr), blood urea nitrogen (BUN), and expression of fibrotic markers. Single-cell RNA sequencing (scRNA-seq) was employed to characterize renal cellular heterogeneity during CKD progression. The results showed that adenine feeding induced renal pathological damage, el

SUBMITTER: Xu Y 

PROVIDER: S-EPMC12748672 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature