Integration of transcriptomics and metabolomics reveals the mechanism of Lycium barbarum polysaccharides inhibited MCF-7 cell proliferation.

ABSTRACT: Breast cancer, as a highly prevalent malignant tumor among women, has a consistently high mortality rate due to metastasis and drug resistance. Lycium barbarum polysaccharides (LBP), which have been reported to have significant anti-tumor activity, have not yet had their molecular mechanism and signaling pathways against breast cancer clearly defined. In this study, the CCK-8 experiment determined that 8 mg/mL LBP treatment for 48 h was the optimal condition for subsequent transcriptomics and metabolomics analyses. The results demonstrated that a high concentration of LBP significantly reduced the viability of MCF-7 cells and inhibited cell proliferation. Transcriptome sequencing revealed that LBP markedly altered the expression levels of the genes HO-1, FTH1, FTL, and TFRC. Metabolomics a

SUBMITTER: Ding L 

PROVIDER: S-EPMC12748708 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature