Synergy between culturomics and metagenomics of health status-associated gut bacteria originating from non-IBD and IBD populations.

ABSTRACT: The bacteria in the human intestinal tract are important for health and associate with diseases, such as inflammatory bowel disease (IBD). Although metagenomic studies can identify certain bacteria or even specific strains and associate their presence or specific phenotypes with health or diseases, actual isolates for experimental validation of metagenomic associations are often lacking. Therefore, this study sets out to culture health- and IBD-associated bacteria from 32 fecal samples from 2 cohorts, for which extensive metadata is available. The cultivation of those samples resulted in 4,347 isolates, of which 1,362 isolates were obtained from IBD patients. Irrespective of health or IBD, Actinomycetota, Bacillota and Bacteroidota were the most represented phyla and members of 5 other phy

SUBMITTER: Plomp N 

PROVIDER: S-EPMC12748711 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature